Device

Part:BBa_K4785011

Designed by: Junfei Xia   Group: iGEM23_SUSTech-CHINA   (2023-10-11)


pBAD-Lysis_E7

It's an induced bacterial suicide device. lysisE7 protein expression was induced by the addition of L-arabinose to the medium, which lyzed the bacteria. By changing the concentration of the inducer, we can explore the optimal conditions for pBAD promoter to induce the expression of the target protein

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 239
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 74
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 56


[edit]
Categories
Parameters
None